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A : 0.24, 2.4 or 24 μg hPBAE, with or without diRNA (at a 40:1 hPBAE:RNA weight ratio), with or without pseudouridin modification (ψ), were delivered to ( A ) Vero, ( B ) Calu-3, or ( C ) A549 cells and cell viability was measured by MTT assay. D : hPBAE–diRNA nanoparticles (0.3 μg of RNA) were delivered to MDBK cells 1 h after exposure to BCoV (MOI of 1), and the amount of BCoV genomic RNA and diRNA in cell extracts and in supernatants were measured after 1 or 24 h.

Journal: bioRxiv

Article Title: Delivery of defective interfering RNA antivirals using hyperbranched poly(beta-amino ester) nanoparticles

doi: 10.64898/2026.05.29.721911

Figure Lengend Snippet: A : 0.24, 2.4 or 24 μg hPBAE, with or without diRNA (at a 40:1 hPBAE:RNA weight ratio), with or without pseudouridin modification (ψ), were delivered to ( A ) Vero, ( B ) Calu-3, or ( C ) A549 cells and cell viability was measured by MTT assay. D : hPBAE–diRNA nanoparticles (0.3 μg of RNA) were delivered to MDBK cells 1 h after exposure to BCoV (MOI of 1), and the amount of BCoV genomic RNA and diRNA in cell extracts and in supernatants were measured after 1 or 24 h.

Article Snippet: Eagle’s Minimum Essential Medium (EMEM) supplemented with 10% FBS was used for human hepatocellular carcinoma HepG2 cells (ATCC #HB-8065), human glioblastoma U-87 MG cells (ATCC #HTB-14), human airway epithelial Calu-3 cells (ATCC #HTB-55), human breast adenocarcinoma MCF-7 cells (ATCC #HTB-22), and bovine kidney epithelial MDBK cells (ATCC #CCL-22).

Techniques: Modification, MTT Assay